FastQCFastQC Report
Sun 19 Mar 2023
SRR4895888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717467
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA2732150.0141.771072
TCTTCAC2736550.0141.543141
TTCACAG848700.0140.976063
TCACAGT287850.0140.210634
TCACATG265600.0139.988824
TTCACAT886850.0139.934273
TCACAGG277200.0138.450064
TCACAGA209650.0138.118744
TCACATA239500.0138.074374
TCACAAG218350.0137.707294
TTCACAC353000.0137.12723
TTCACAA694900.0136.979223
TCACACG87650.0136.358354
TCACATT293900.0135.408664
TCACAAT242250.0135.35964
TCACAGC88900.0134.441074
TCACATC105750.0132.900684
CACATAG81850.0132.614145
CACAGTA82450.0131.994865
CACAGTG97150.0131.829885
Produced by FastQC (version 0.11.5)