FastQCFastQC Report
Sun 19 Mar 2023
SRR4895888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717467
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG482601.775918530013428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC287121.0565721681256846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86530.31842153004985896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC83320.30660905909804975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT48840.17972619354715255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT44940.16537459332532833No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA38880.14307441451910916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA31470.11580637409764315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC29690.10925615656050285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC28650.1054290631680164No Hit
GTGAACCCCGTGGTCTCCTACGCCGTGGCTCTCAGCTGTCAATGTGCACT27910.10270593902336257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT909450.0140.961791
GGTATCA921800.0139.370968
GTGGTAT922500.0139.054476
TGGTATC924200.0138.97017
GTATCAA925950.0138.824079
CAGTGGT932150.0137.6694
AGCAGTG931200.0137.622942
GCAGTGG940600.0136.285873
AGTGGTA943500.0135.997635
CAACGCA919450.028.62241210-14
TCAACGC920250.028.57562310-14
ATCAACG919750.028.55045110-14
AGAGTAC919500.028.49463515-19
CGCAGAG920950.028.45133415-19
TACATGG919050.028.39535720-24
GTACATG920250.028.37554420-24
CAGAGTA925250.028.3237815-19
AGTACAT922950.028.2738120-24
GCAGAGT929000.028.17379615-19
AACGCAG943100.027.90770510-14
Produced by FastQC (version 0.11.5)