FastQCFastQC Report
Sun 19 Mar 2023
SRR4895887_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895887_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857205
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA858250.0142.364352
CGTCTAG262350.0142.239913
CGTCTAA215650.0142.208253
TCCGTCT861500.0141.827291
CGTCTAT283550.0141.784683
GTCTAAG71850.0141.150044
GTCTAGA63550.0141.080814
CGTCTAC99300.0140.959753
GTCTATA76050.0140.383324
GTCTATG93750.0140.18214
GTCTAGT90650.0140.180114
GTCTAAT72900.0139.801364
GTCTACG26650.0139.597054
GTCTAGG87750.0139.294
GTCTAGC24650.0138.78014
GTCTATC30900.0137.233894
GTCTAAA59150.0136.634774
GTCTACC30550.0136.006684
GTCTATT89550.0135.614734
TCTAGTA25450.0135.257775
Produced by FastQC (version 0.11.5)