FastQCFastQC Report
Sun 19 Mar 2023
SRR4895887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857205
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG166481.9421258625416322No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96221.1224852864833967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT47470.5537765178691211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT38590.45018402832461313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT33210.3874219119113864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC30380.3544076387795218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26510.3092609119172193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA24750.28872906714263213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC24020.28021301788953634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT23050.2688971716217241No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT21400.2496485671455486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA14800.1726541492408467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG13850.16157161939092748No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC11440.13345699103481665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG11350.13240706715429798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA11250.1312404850648328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACCCGTCTATGTA10980.1280907134232768No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC10130.11817476566282278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAATAGATATAGTACCG9810.11444170297653421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC9720.11339177909601554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA9170.10697557760395704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGTAGCCTGGAGGCTCCG9120.10639228655922445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT292350.0140.923581
GGTATCA294050.0140.125098
GTATCAA294950.0139.721929
GTGGTAT295100.0139.455726
TGGTATC296250.0139.10887
CAGTGGT297900.0138.241624
GCAGTGG298600.0137.925663
AGCAGTG300400.0137.12322
AGTGGTA302600.0136.070665
GACTGGA5200.030.4779683
ATCAACG293650.028.61729810-14
CAACGCA294200.028.60295510-14
AGAGTAC293950.028.53420615-19
TACATGG293250.028.46482820-24
CGCAGAG294650.028.44198215-19
TCAACGC297200.028.29484610-14
CAGAGTA296050.028.28316115-19
GTACATG295850.028.22927520-24
AGTACAT296650.028.15820-24
ACATGGG296450.028.10899720-24
Produced by FastQC (version 0.11.5)