FastQCFastQC Report
Sun 19 Mar 2023
SRR4895886_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895886_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387735
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC3394100.0142.269581
ATCAGCA3394300.0142.261182
TCAGCAT1068650.0141.838093
TCAGCAG1073650.0141.741783
TCAGCAA851450.0141.374883
TCAGCAC417100.0140.846743
CAGCAGT364050.0139.934634
CAGCATG341750.0139.681324
CAGCATA283950.0139.526444
CAGCAAT297400.0139.063554
CAGCATT348500.0138.673234
CAGCAAG275700.0138.195514
CAGCAGG364550.0137.787724
CAGCAGA264500.0137.472724
AGCAATG98500.0135.737015
AGCATGG117300.0135.429665
AGCATTG118600.0135.387395
AGCATGT120000.0135.352025
CAGCATC117500.0135.25984
AGCAGTG121750.0135.104115
Produced by FastQC (version 0.11.5)