FastQCFastQC Report
Sun 19 Mar 2023
SRR4895885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104564
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC205061.856479117552265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG47480.42985286502185477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36350.3290891247587283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA36290.3285459240025929No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG35750.3236571171973738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28890.2615511640792204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT26340.23846513194346364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA24260.21963417239743463No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG21800.1973629413958811No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG20280.18360185557378297No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT17680.16006315614124667No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA17210.15580808355151896No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG16860.15263941247406215No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT16720.15137194404307944No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT16550.149832875234029No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT16020.14503460188816583No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC15930.14421980075396265No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT15690.14204699772942084No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT15420.1396025943268113No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG14660.13272205141576224No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC14600.13217885065962678No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT14580.13199778374091495No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA14210.12864804574474634No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC14200.12855751228539042No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT14150.12810484498861088No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC13880.12566044158600134No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT13780.12475510699244227No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT13620.12330657164274772No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT13550.12267283742725638No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT13520.12240123704918864No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG13520.12240123704918864No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA13440.12167696937434137No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC13190.11941363289044364No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT13160.11914203251237591No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT13060.11823669791881683No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC13050.11814616445946093No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG13000.11769349716268139No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA12930.11705976294719002No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA12800.11588282797556321No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA12800.11588282797556321No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT12720.11515856030071593No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG12660.11461535954458049No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT12620.11425322570715686No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT12550.11361949149166549No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC12510.11325735765424186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT12350.1118088223045473No Hit
CTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGA12330.1116277553858355No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC12320.1115372219264796No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT12200.11045082041420869No Hit
GTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGA12130.10981708619871731No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT12090.10945495236129368No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT12060.10918335198322597No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG11880.1075537497148196No Hit
ATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTG11870.10746321625546369No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG11810.10692001549932825No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT11740.10628628128383688No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA11740.10628628128383688No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA11710.10601468090576915No Hit
TGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACT11590.10492827939349826No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT11460.10375134442187145No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG11410.10329867712509189No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG11390.10311761020638008No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG11290.10221227561282098No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG11290.10221227561282098No Hit
AGTAATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTA11290.10221227561282098No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC11280.10212174215346508No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC11270.10203120869410917No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT11190.1013069410192619No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC11090.1004016064257028No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT11090.1004016064257028No Hit
AATGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTT11070.10022053950699099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT238150.0140.502931
GGTATCA239600.0139.578348
GTGGTAT239750.0139.430956
GTATCAA240050.0139.346689
TGGTATC241650.0138.424047
AGTGGTA243300.0137.514865
CAGTGGT244150.0137.065614
AGCAGTG244450.0136.734562
GCAGTGG246800.0135.520133
CAACGCA239100.028.5883310-14
ATCAACG238650.028.58189810-14
AGAGTAC239350.028.40806615-19
TACATGG237950.028.3883820-24
GTACATG238400.028.37103820-24
CGCAGAG240650.028.3623115-19
CAGAGTA240650.028.26657315-19
TCAACGC241850.028.23348810-14
AGTACAT239850.028.22353620-24
GCAGAGT243100.028.01723715-19
AACGCAG244400.027.98015410-14
Produced by FastQC (version 0.11.5)