FastQCFastQC Report
Sun 19 Mar 2023
SRR4895884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076620
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG110271.0242239601716483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC67850.6302130742508963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC63640.5911092121639947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50990.4736118593375564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA32480.30168490275120285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA31410.29174639148446063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG26330.2445616837881518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA24850.23081495792387288No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT23300.21641804907952666No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG16640.1545577826902714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC16440.15270011703293643No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG14810.13756014192565622No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT13680.1270643309617135No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC12500.11610410358343705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA12280.11406067136036857No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT12240.11368913822890156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA12080.11220300570303357No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG11990.11136705615723282No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG11890.11043822332856533No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT11390.10579405918522784No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT11320.10514387620516058No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT11290.10486522635656036No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT11130.10337909383069237No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA11010.10226449443629135No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT10850.10077836191042336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT297600.0140.433471
GGTATCA299050.0139.906238
GTGGTAT298950.0139.856696
GTATCAA299900.0139.53379
TGGTATC302050.0138.540517
AGCAGTG303300.0137.794272
AGTGGTA304250.0137.467735
GCAGTGG305850.0136.810243
CAGTGGT305800.0136.794514
CAACGCA298350.028.65503710-14
ATCAACG298850.028.60227610-14
CGCAGAG298600.028.5587115-19
TACATGG296600.028.55708320-24
GTACATG297800.028.47585920-24
CAGAGTA298300.028.46674715-19
TCAACGC301050.028.40760610-14
AGTACAT298300.028.40399220-24
AGAGTAC300050.028.31991615-19
GCAGAGT302800.028.05320515-19
AACGCAG304700.028.0484110-14
Produced by FastQC (version 0.11.5)