FastQCFastQC Report
Sun 19 Mar 2023
SRR4895883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188514
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGTC3197800.0142.123031
TCTGTCA3198500.0142.091922
CTGTCAG975900.0141.661213
CTGTCAA820050.0141.598973
CTGTCAT1044800.0141.487093
TGTCAGT318450.0139.471534
TGTCATG340250.0139.458534
TGTCAAT279850.0139.354234
TGTCATA286350.0139.028274
TGTCAAG265250.0138.910324
TGTCAGG327500.0138.838124
TGTCAGA255900.0138.248634
GTCATAG95900.0136.889575
GTCAGTA93250.0136.881935
GTCAATG93400.0136.66215
GTCATGG119400.0136.449285
GTCAGAT86600.0136.447635
GTCATGA86650.0135.299675
TGTCAGC96100.0135.121464
GTCAATA78350.0134.987725
Produced by FastQC (version 0.11.5)