FastQCFastQC Report
Sun 19 Mar 2023
SRR4895883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188514
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG358301.1237209559061054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC261500.8201312586364683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC216640.6794387605009732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112180.35182533305483366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG87790.2753320198688166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA87200.2734816281189294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA68830.2158685832961687No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG50620.15875733962591979No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG46570.1460554979529649No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA39180.12287855722132629No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT38950.1221572180645906No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG37300.11698239367931268No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT35910.11262299616686645No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT35680.11190165701013073No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC35490.11130576814152297No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT35070.1099885401161795No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT34320.10763634721378046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA34300.10757362206971649No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG33590.10534687945544538No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA32780.10280651112085443No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT32720.10261833568866251No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT32280.10123838251925506No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT31960.10023478021423146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT770400.0140.528321
GGTATCA778750.0139.28198
GTGGTAT780300.0138.866826
TGGTATC782500.0138.58687
GTATCAA782950.0138.562339
AGCAGTG788650.0137.239852
CAGTGGT791000.0137.070274
AGTGGTA791000.0137.024755
GCAGTGG801350.0135.163713
CAACGCA777800.028.59761610-14
ATCAACG777300.028.54561210-14
TCAACGC778750.028.54053910-14
AGAGTAC775350.028.46140515-19
TACATGG772450.028.43030720-24
CGCAGAG781350.028.35710715-19
GTACATG776600.028.330320-24
AGTACAT779300.028.19703520-24
CAGAGTA783150.028.18344915-19
GCAGAGT784750.028.11681215-19
AACGCAG791200.028.10963610-14
Produced by FastQC (version 0.11.5)