FastQCFastQC Report
Sun 19 Mar 2023
SRR4895882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715684
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG716650.0142.34691
TCGTAGA716850.0142.307172
CGTAGAT238000.0142.276283
CGTAGAG216800.0141.88833
CGTAGAA183450.0141.496813
CGTAGAC81050.0141.050863
GTAGACG20450.0139.060584
GTAGAGT70350.0138.695954
GTAGATG73550.0138.572744
GTAGATA67950.0137.511064
GTAGAAT70150.0137.059364
GTAGAAG58950.0136.984224
GTAGAGA54800.0136.693084
GTAGAGC26100.0136.538124
GTAGACC26700.0136.406194
GTAGATT73650.0135.86854
GTAGAGG73300.0135.73944
GTAGACT20900.0135.38444
TAGACGA4200.0132.363955
TAGATAG23150.0132.078085
Produced by FastQC (version 0.11.5)