FastQCFastQC Report
Sun 19 Mar 2023
SRR4895882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715684
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG103091.440440194275686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC62730.8765041554652612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA27240.38061490825559885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT26890.3757244817545174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC24600.3437271197902985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT18510.25863369867148067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18040.25206655451288557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15590.21783356900531517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC14040.1961759659290972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA10830.151323768590607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC8960.12519491842768596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA8470.11834832132617187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC8400.11737023602595559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG8350.11667160366865822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC7880.11010445951006309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT178850.0139.174671
GGTATCA179600.0138.825678
GTGGTAT179800.0138.4316
GTATCAA180500.0138.133479
TGGTATC182000.0136.836767
AGCAGTG182200.0136.536682
CAGTGGT182700.0136.31254
GCAGTGG184800.0134.77163
AGTGGTA185350.0134.36365
CAACGCA179350.028.52643410-14
ATCAACG179950.028.37530510-14
AGAGTAC180250.028.36802115-19
GTACATG178950.028.34879320-24
CAGAGTA180750.028.28954715-19
TACATGG179100.028.28485120-24
AGTACAT179600.028.25421320-24
CGCAGAG180850.028.22613115-19
TCAACGC182150.028.11954710-14
GCAGAGT182600.027.96350515-19
GAGTACA178750.027.71188420-24
Produced by FastQC (version 0.11.5)