FastQCFastQC Report
Sun 19 Mar 2023
SRR4895881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985081
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA985900.0142.430011
TACGCAA986350.0142.365042
ACGCAAT323250.0142.001683
CGCAATA83950.0141.449174
CGCAAGT105100.0141.196564
CGCAATG104700.0140.782934
ACGCAAG319350.0140.700413
ACGCAAA229900.0140.010653
CGCAATT97900.0139.859124
ACGCAAC124950.0139.244373
CGCAAGG111850.0138.729424
CGCAAGA75100.0138.18714
GCAAGTA28600.0138.066995
GCAATTG35700.0136.363655
CGCAACG34100.0136.073244
CGCAATC41150.0135.970894
GCAAGTG37000.0135.810555
CGCAAAT82950.0134.733574
GCAAGAT27800.0134.604835
CGCAAAG79700.0134.414694
Produced by FastQC (version 0.11.5)