FastQCFastQC Report
Sun 19 Mar 2023
SRR4895881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985081
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG145611.4781525580129957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC88120.8945457277117314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC43280.4393547332655894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23640.23998026558222116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG23570.23926966411899125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22630.22972730161276078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21400.21724101875886348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT12660.12851735034986972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10600.10760536443196042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC10380.1053720455475235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA10260.10415387161055792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA235400.0140.05418
AAGCAGT235450.0139.855061
GTGGTAT237000.0139.04786
GTATCAA237650.0138.818989
TGGTATC239050.0137.85547
CAGTGGT241250.0136.747484
AGCAGTG240700.0136.744782
AGTGGTA243800.0135.169535
GCAGTGG244700.0134.627183
CGATAGG255.3699425E-428.7999435-39
CAACGCA236050.028.4827210-14
CAGAGTA236200.028.37318415-19
AGAGTAC236050.028.37291315-19
ATCAACG236850.028.35611510-14
GTACATG235700.028.31729120-24
TCAACGC238600.028.21452510-14
TACATGG236600.028.2034920-24
AGTACAT236750.028.1795420-24
CGCAGAG238350.028.10516515-19
GAGTACA235300.027.87584520-24
Produced by FastQC (version 0.11.5)