FastQCFastQC Report
Sun 19 Mar 2023
SRR4895880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099324
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA1100700.0142.357772
GGTGCGA1101000.0142.3191
TGCGAAG351600.0141.890433
TGCGAAT360250.0141.727953
TGCGAAA263500.0141.565383
TGCGAAC130500.0141.33763
GCGAATA99600.0141.036394
GCGAATG115200.0140.744954
GCGAAGT113500.0140.404134
GCGAATC47150.0140.271744
CGAAGTA31500.0139.371545
GCGAAGG119100.0138.888844
CGAATAT35100.0138.681185
GCGAATT102400.0138.502334
GCGAAGA89900.0138.337424
GCGAACT32950.0137.780564
CGAAGGT38800.0137.579595
CGAATCA12450.0137.387055
CGAATGG40900.0136.962975
GCGAAAG94500.0136.731924
Produced by FastQC (version 0.11.5)