FastQCFastQC Report
Sun 19 Mar 2023
SRR4895879_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895879_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296164
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG100.0074563813142.565698
ACATCGA1000.0142.541616
GGAGAAC297550.0141.870941
GAGAACA297750.0141.775652
GAACAGT33600.0140.632574
AGAACAG96650.0140.624343
AGAACAT92750.0140.543733
GAACAAT24250.0139.602624
GAACATG29450.0137.459494
GAACATT27950.0137.441744
AGAACAA77850.0136.774033
GAACAGA24500.0136.141784
GAACAGG31550.0135.538774
AGAACAC36550.0135.131793
GAACATA27800.0135.106894
GAACACG9450.0133.491354
GAACATC10450.0132.993384
AACATCG3650.0132.77855
GAACAGC9450.0132.737174
GAACACT9650.0131.463264
Produced by FastQC (version 0.11.5)