FastQCFastQC Report
Sun 19 Mar 2023
SRR4895879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296164
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35181.1878553774260205No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32341.091962561283613No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21720.7333774530327791No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA13910.46967220864115833No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA12750.4305047203576397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12090.4082197701273618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC4610.1556570008508799No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3340.11277535419564835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65150.0139.458948
GTGGTAT65300.0139.028346
GTATCAA65750.0138.29589
AAGCAGT66050.0138.1271
TGGTATC66100.0137.345697
AGCAGTG66600.0136.770072
CAGTGGT67100.0135.620714
GCAGTGG67250.0135.233983
AGTGGTA69700.0130.14855
CTTAAGG800.002105327835.9974254
CGATAGG255.3660886E-428.8018335-39
CGTCGCG403.4605728E-728.798914130-134
CGTACGG403.4615914E-728.79794150-54
CAACGCA65500.028.53414210-14
ATCAACG65200.028.53292810-14
TACATGG65000.028.42423220-24
AGAGTAC65600.028.29309815-19
GTACATG65900.028.0578920-24
GCAGAGT66400.027.97390215-19
TCAACGC66950.027.93765810-14
Produced by FastQC (version 0.11.5)