FastQCFastQC Report
Sun 19 Mar 2023
SRR4895878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842815
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA2845700.0142.39752
GCTCGGT2849000.0142.232571
TCGGTAA709350.0142.04043
TCGGTAG897650.0141.939353
TCGGTAC360800.0141.910663
TCGGTAT888550.0141.63643
CGGTAGA222950.0141.168184
CGGTATA239600.0141.055484
CGGTAAG236750.0140.616124
CGGTATG301050.0140.31744
CGGTAAT236200.0140.159014
CGGTAGT291750.0140.099884
CGGTACG95550.0139.633744
CGGTACA70150.0138.986834
CGGTAGC88150.0138.661874
CGGTACC110400.0138.604784
CGGTACT92700.0138.160394
CGGTAGG310300.0138.063924
CGGTAAA189500.0137.653444
CGGTATC93150.0136.804344
Produced by FastQC (version 0.11.5)