FastQCFastQC Report
Sun 19 Mar 2023
SRR4895877_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895877_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618524
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA1620200.0142.394062
GCTAACG1620550.0142.363311
TAACGAG530250.0142.126943
TAACGAT503350.0142.076353
TAACGAC190600.0141.384433
TAACGAA402450.0141.339373
AACGATG168600.0140.937264
AACGATA139950.0140.455634
AACGAGT159200.0140.349954
AACGAGG189500.0139.121064
AACGATT152300.0138.940254
AACGAAT136200.0138.618964
AACGACT48400.0138.125954
AACGAGA134000.0137.863084
AACGATC50350.0137.730824
ACGATGG58850.0137.699335
AACGAAG139500.0137.638894
ACGATAT47250.0137.56595
ACGATTG51000.0137.512665
ACGATGT56650.0137.511185
Produced by FastQC (version 0.11.5)