FastQCFastQC Report
Sun 19 Mar 2023
SRR4895877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618524
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG170691.05460283567003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC95910.5925769404716891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC43710.2700608702743982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43010.2657359421299901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC40860.2524522342578794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT39860.2462737654801535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA30990.19147074742172498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT27150.1677454273152576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA25580.158045231334228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT22990.14204299719991795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC19320.11936801678566397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA342800.0138.953358
GTATCAA344150.0138.387349
GTGGTAT344350.0138.244266
AAGCAGT344800.0137.990131
TGGTATC345800.0137.789497
CAGTGGT349750.0136.274494
AGCAGTG353900.0134.421592
GCAGTGG355600.0133.941013
AGTGGTA356900.0133.443565
CAACGCA341050.028.5243610-14
ATCAACG341600.028.4109910-14
TCAACGC342600.028.37009210-14
AGAGTAC341250.028.36839715-19
TACATGG341550.028.22999620-24
GTACATG342050.028.2224120-24
CGCAGAG343900.028.21260315-19
AGTACAT344100.028.0333520-24
CAGAGTA345750.027.9950115-19
GAGTACA340450.027.8263520-24
GCAGAGT349700.027.66644515-19
Produced by FastQC (version 0.11.5)