FastQCFastQC Report
Sun 19 Mar 2023
SRR4895876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5031744
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA5036150.0142.425322
GCGAGTA5036700.0142.409771
GAGTAAC606500.0141.49363
GAGTAAT1641550.0141.452763
GAGTAAG1660450.0141.177673
AGTAACG173550.0139.142534
AGTAATG558150.0138.707524
GAGTAAA1192900.0138.183563
AGTAAGT559850.0137.70074
AGTAATC219650.0137.521594
AGTAAGG586600.0136.755424
AGTAAGA406950.0136.631354
AGTAATA433800.0136.471984
AGTAACC196900.0136.361284
AGTAAGC158350.0135.799564
AGTAACT145500.0135.546194
AGTAATT486500.0134.376194
AGTAAAT421400.0134.24654
GTAATAG157200.0132.349625
GTAATGA138850.0131.925385
Produced by FastQC (version 0.11.5)