SRR4895875_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR4895875_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41026
Sequences flagged as poor quality	0
Sequence length	150
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG	619	1.5087992980061424	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC	351	0.8555550138936284	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCAGGCCGTTTGGCATCTC	263	0.641056890752206	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA	192	0.46799590503583094	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC	175	0.42655876761078343	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	158	0.38512163018573586	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA	130	0.3168722273680105	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA	106	0.25837273923853166	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG	91	0.2218105591576074	No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG	65	0.15843611368400526	No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG	64	0.15599863501194364	No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT	52	0.1267488909472042	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTTCAAAGTGGAGA	50	0.12187393360308096	No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCTCAATCCGCAAAAAAAAAAAAA	50	0.12187393360308096	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTTACTGAGAACTCATAA	48	0.11699897625895773	No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT	46	0.11212401891483449	No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG	45	0.10968654024277287	No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT	45	0.10968654024277287	No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC	44	0.10724906157071126	No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT	44	0.10724906157071126	No Hit
GGCGTGAACCCCGTGGTCTCCTACGCCGTGGCTCTCAGCTGTCAATGTGC	42	0.10237410422658802	No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT	42	0.10237410422658802	No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC	42	0.10237410422658802	No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT	42	0.10237410422658802	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	870	0.0	141.51724	1
GTGGTAT	880	0.0	139.90909	6
TGGTATC	880	0.0	139.90909	7
GGTATCA	885	0.0	138.30508	8
AGCAGTG	900	0.0	136.8	2
CAGTGGT	905	0.0	136.0442	4
GCAGTGG	920	0.0	133.82608	3
AGTGGTA	920	0.0	133.82608	5
GTATCAA	920	0.0	133.04347	9
TGGAGTT	30	0.001975992	72.00001	6
CCATCTC	30	0.001975992	72.00001	4
AGTTCAG	40	0.0061765467	54.0	9
CTGGAGT	40	0.0061765467	54.0	5
GTGACTG	45	0.009839193	48.0	1
TGACTGG	45	0.009839193	48.0	2
CGTGGGT	35	3.963285E-6	28.800001	60-64
ACTGACC	35	3.963285E-6	28.800001	100-104
TTTCGAC	35	3.963285E-6	28.800001	65-69
GGTTTCG	35	3.963285E-6	28.800001	60-64
ATCGTGG	35	3.963285E-6	28.800001	55-59

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content