FastQCFastQC Report
Sun 19 Mar 2023
SRR4895874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10803862
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG1434000.0141.760254
GAACAAT3900950.0141.373143
AACAAGT1418550.0140.431444
AACAAGG1645700.0140.324554
ACAATGG521850.0140.260595
ACAAGTG485700.0140.23435
ACAATTG476500.0140.055835
ACAATAG408500.0139.134965
ACAAGTA427300.0138.6755
ACAATGA401800.0136.796025
CAATTAG150000.0135.83966
CAAGTAG154250.0135.784366
AACAATT1330500.0135.76874
CAATAGG156550.0135.353166
ACAAGAG425650.0135.322055
CAAGTAT142900.0134.879946
AACAATA1150800.0134.86514
CAAGTTG166400.0134.610186
CAAGTGG179950.0134.516976
AACAAAG1175450.0134.493124
Produced by FastQC (version 0.11.5)