FastQCFastQC Report
Sun 19 Mar 2023
SRR4895873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583647
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG2593750.0143.435941
GAAGAGA2599700.0143.107642
AAGAGAT884950.0142.494283
AAGAGAG997000.0141.623523
AAGAGAA732150.0140.852543
AGAGATG374450.0129.174564
GAGATGA100600.0128.110845
AGAGATA279750.0127.70784
AGAGAGG425550.0127.689314
AGAGAGT351300.0126.4350364
AGAGAAG309400.0126.4067464
AGAGAAT269300.0125.952724
AGAGAGA302100.0125.7671364
GAGATTG116400.0125.504675
GAGATAG104350.0125.2318955
GAGAGTA102450.0125.1649555
GAGAGTG127600.0124.5324255
GAGAATA74100.0124.469155
GAGATTA88900.0124.399975
GAGATGG146550.0124.1515
Produced by FastQC (version 0.11.5)