FastQCFastQC Report
Sun 19 Mar 2023
SRR4895873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583647
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG54220.2098583900974088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC48710.18853194728227193No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG46750.1809457716166334No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG42880.16596694517478586No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC41840.16194162747465113No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG41310.15989026364669787No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA40230.1557101260350195No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC39800.15404581198592532No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT38190.14781431054629368No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC37850.14649834129817269No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT37280.14429215755867578No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC37160.14382769782404486No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC36790.14239561364226613No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT36670.1419311539076352No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT35350.13682209682669497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT35030.13558353753434582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC34970.13535130766703035No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT34650.13411274837468123No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT34470.13341605877273482No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT34300.13275807414867433No Hit
TGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGC33970.13148080987843927No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT33850.13101635014380833No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT33810.1308615302322647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33760.13066800534283515No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC33150.1283070016917946No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT33000.12772642702350592No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT32710.12660398266481449No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC32260.12486225865994852No Hit
TACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCT32200.12463002879263305No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA32070.12412686408011621No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC32030.12397204416857256No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG31810.12312053465508253No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC31630.12242384505313612No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA31250.12095305589347152No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC30670.11870816717608869No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT30480.11797277259625637No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT30480.11797277259625637No Hit
AAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGA30420.11774054272894093No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC30200.11688903321545087No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA30090.11646327845870585No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA29550.11437320965286668No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT29410.11383133996246392No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC29380.11371522502880618No Hit
GGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCT29240.11317335533840343No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT28840.111625156222967No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA28800.11147033631142335No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG28700.11108328653256425No Hit
AACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAA28590.11065753177581922No Hit
AGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCG28490.1102704819969601No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA28460.11015436706330237No Hit
CACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTT28430.11003825212964466No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA28390.10988343221810101No Hit
CGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAA28250.10934156252769825No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT28240.10930285754981234No Hit
TTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA28090.10872228288152368No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT28090.10872228288152368No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA27990.10833523310266456No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC27950.10818041319112091No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA27770.10748372358917453No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA27700.10721278874397315No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT27590.10678703398722814No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC27390.1060129344295099No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT27150.10508401496024805No Hit
ACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTA26990.10446473531407349No Hit
CGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAACTC26870.10400027557944255No Hit
TGGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGC26720.10341970091115389No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC26620.10303265113229478No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA26590.10291653619863704No Hit
ACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCC26490.10252948641977792No Hit
TCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATG26420.10225855157457657No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG26220.10148445201685834No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC26140.10117481219377106No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG26070.10090387734856968No Hit
AGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTC26040.10078776241491194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216100.0140.800178
GTGGTAT217350.0139.990436
TGGTATC217350.0139.990437
AAGCAGT218550.0139.262191
GTATCAA221950.0137.056639
CAGTGGT222350.0136.82334
AGCAGTG225950.0134.677812
GCAGTGG228850.0133.03413
AGTGGTA236900.0128.43785
TCAACGC214850.028.59866310-14
ATCAACG214850.028.5584510-14
CAACGCA217450.028.30307210-14
AGAGTAC216600.028.28782715-19
TACATGG216150.028.21347820-24
CGCAGAG217500.028.20387515-19
GTACATG216900.028.1358420-24
CAGAGTA218050.028.13273615-19
GAGTACA215900.028.11275520-24
AGTACAT217950.028.01350420-24
TATCAAC221900.027.52132810-14
Produced by FastQC (version 0.11.5)