FastQCFastQC Report
Sun 19 Mar 2023
SRR4895872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385994
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA3404350.0143.221652
TTCACAG1249400.0143.175873
TCTTCAC3413600.0142.833541
TCACAGT420750.0142.802084
TCACATG432850.0142.436364
TCACAGA336150.0142.093644
TTCACAT1181600.0141.940373
TCACATA337150.0141.181024
TCACAAG362100.0140.977574
CACATGG159550.0140.795935
CACATAG125150.0140.77825
CACATTG143500.0140.537935
CACAGTA118750.0140.48335
CACAGTG153250.0140.335345
TCACAAT342050.0139.916344
TTCACAA993250.0139.824553
CACAGAT110350.0139.367425
TCACATT390650.0139.355384
CACATGT145900.0138.9175
CACAAGT122450.0138.531595
Produced by FastQC (version 0.11.5)