FastQCFastQC Report
Sun 19 Mar 2023
SRR4895872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385994
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGC89800.26521015689927385No Hit
AAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGA76000.22445403033791553No Hit
GGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCT74240.21925614753009015No Hit
CGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAA73890.21822247765353395No Hit
AACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAA72500.21411733157235363No Hit
TGGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGC69800.20614330681034873No Hit
AGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCG68050.20097495742756782No Hit
GAGACATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGG67640.19976408700074483No Hit
CGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAACTC67630.19973455357570036No Hit
AGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACAC66720.19704701189665424No Hit
TCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATG61510.18166009744848927No Hit
AAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTC60660.17914975631970997No Hit
ACAACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGG59610.1760487466900414No Hit
GCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGA59570.17593061298986354No Hit
ACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACA58240.17200266745895001No Hit
CATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGA57850.170850863882216No Hit
GATGACTATCACCTCCTAAAGGACCTAGAGGAAGGCATCCAAACGCTGAT57590.17008299483105996No Hit
GGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGA56060.16556438079925717No Hit
GCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCA54520.16101623334240994No Hit
GGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGC53300.1574131554869855No Hit
AAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGAGGGCAG53240.15723595493671874No Hit
CAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCG52110.15389867790669445No Hit
CTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGG51580.15233340637933795No Hit
CTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCAT50990.15059093430171466No Hit
CACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGAC50840.15014793292604772No Hit
ATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGAC50760.14991166552569202No Hit
AGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGTGCCCGAGTAGCAT50580.1493800638748917No Hit
ATCCAAACGCTGATGGGGAGGCTGGAAGACGGCAGCCGCCGGACTGGGCA50390.1488189287990469No Hit
TGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCA50170.1481691934480687No Hit
AGACCTACAGCAAGTTTGACACAAACTCGCACAACCATGACGCACTGCTC49880.1473127241217793No Hit
GGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAACTCG49310.14562931889424494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49270.14551118519406708No Hit
ATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGA49190.1452749177937114No Hit
CAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCC48730.14391638024166611No Hit
AGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTCT47610.1406086366366863No Hit
TACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGT46810.1382459626331293No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG46120.13620815630506136No Hit
AGCTGTGGCTTCTAGGTGCCCGAGTAGCATCCTGTGACCCCTCCCCAGTG45640.13479055190292719No Hit
CACAACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAG45540.13449521765248254No Hit
GACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACAT45480.13431801710221578No Hit
CGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGG45320.13384548230150436No Hit
CGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGTGCCCGAGTAGCATCCTGT45280.1337273486013265No Hit
CTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACAT44710.13204394337379216No Hit
CTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGT44660.13189627624856984No Hit
AGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAG44070.13015380417094657No Hit
CTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCG43810.12938593511979052No Hit
CGAGACATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTG43770.12926780141961267No Hit
ACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTG43750.12920873456952375No Hit
GGACAGCGATGACTATCACCTCCTAAAGGACCTAGAGGAAGGCATCCAAA43540.12858853264359002No Hit
AGCGATGACTATCACCTCCTAAAGGACCTAGAGGAAGGCATCCAAACGCT43500.12847039894341217No Hit
GCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGG43460.12835226524323434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43440.1282931983931454No Hit
GAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCT43230.1276729964672117No Hit
CAACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGA43180.1275253293419894No Hit
AAACTCGCACAACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACT43050.12714139481641137No Hit
ATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGTGCCCGA43010.12702326111623352No Hit
TGATGGGGAGGCTGGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCAAG42720.1261667917899441No Hit
GGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTG42630.12590099096454394No Hit
CTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGC42050.12418805231196513No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG41910.12377458436134264No Hit
CTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGT41700.12315438243540892No Hit
GGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCA41060.12126424323256331No Hit
TCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTC40970.12099844240716316No Hit
GCTCTGTGGAGGGCAGCTGTGGCTTCTAGGTGCCCGAGTAGCATCCTGTG40880.120732641581763No Hit
AGAGGAAGGCATCCAAACGCTGATGGGGAGGCTGGAAGACGGCAGCCGCC40680.12014197308087374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC40470.11952177115494003No Hit
CGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCC40420.11937410402971772No Hit
AACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGAC40290.1189901695041397No Hit
AGACATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGC40250.11887203580396184No Hit
ATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCT40110.11845856785333937No Hit
AAACGCTGATGGGGAGGCTGGAAGACGGCAGCCGCCGGACTGGGCAGATC40050.1182813673030726No Hit
AGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAACTCGCACAACCAT39920.11789743277749458No Hit
AAAGGACCTAGAGGAAGGCATCCAAACGCTGATGGGGAGGCTGGAAGACG39500.11665702892562715No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC39020.11523942452349295No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG39000.11518035767340404No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA38830.11467828944764817No Hit
GGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTCTG38110.11255188284444687No Hit
CTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAA38080.11246328256931347No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC37940.112049814618691No Hit
CCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGG37670.1112524121424905No Hit
ACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACA37660.11122287871744604No Hit
GCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAAC37430.1105436099414234No Hit
TGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAG37430.1105436099414234No Hit
CGCATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGTGCC37180.10980527431531184No Hit
GGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGAGG37020.10933273951460043No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT36760.10856487046344442No Hit
AAGGACCTAGAGGAAGGCATCCAAACGCTGATGGGGAGGCTGGAAGACGG36760.10856487046344442No Hit
AGACGGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGT36660.1082695362129998No Hit
AAGGCATCCAAACGCTGATGGGGAGGCTGGAAGACGGCAGCCGCCGGACT36380.10744260031175484No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT36380.10744260031175484No Hit
CTAGAGGAAGGCATCCAAACGCTGATGGGGAGGCTGGAAGACGGCAGCCG36290.10717679948635467No Hit
CAGACCTACAGCAAGTTTGACACAAACTCGCACAACCATGACGCACTGCT36280.10714726606131021No Hit
AAGCAGACCTACAGCAAGTTTGACACAAACTCGCACAACCATGACGCACT36240.10702913236113236No Hit
CATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGTGCCCG35710.10546386083377585No Hit
CAAACGCTGATGGGGAGGCTGGAAGACGGCAGCCGCCGGACTGGGCAGAT35650.10528666028350907No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC35550.10499132603306444No Hit
GCACAACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCA35380.10448925780730858No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT35120.10372138875615255No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC34950.10321932053039667No Hit
GCATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGTGCCC34720.10254005175437404No Hit
GCTGATGGGGAGGCTGGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCA34570.1020970503787071No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT34470.10180171612826248No Hit
GACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGCTCTGT34400.10159498215295125No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT34240.10112244735223984No Hit
GGGGAGGCTGGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGA34010.10044317857621719No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT33950.10026597802595043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA262050.0140.647428
TGGTATC264000.0139.826747
GTGGTAT263850.0139.824376
AAGCAGT264200.0139.739441
CAGTGGT267950.0137.775654
AGCAGTG271800.0135.824082
GCAGTGG273300.0135.104953
AGTGGTA273650.0134.869575
GTATCAA278000.0132.50029
CAACGCA260900.028.57359510-14
TCAACGC261200.028.53526310-14
ATCAACG261000.028.52402510-14
AGAGTAC262100.028.39332615-19
CAGAGTA263000.028.33996815-19
TACATGG262750.028.32856620-24
CGCAGAG263950.028.22705315-19
GTACATG264450.028.17368320-24
AGTACAT266500.027.92454520-24
GAGTACA263900.027.91046320-24
GCAGAGT267150.027.89972315-19
Produced by FastQC (version 0.11.5)