FastQCFastQC Report
Sun 19 Mar 2023
SRR4895871_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895871_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4804967
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA4812250.0143.781162
CGTCTAG1767100.0143.641043
CGTCTAA1411850.0143.586533
CGTCTAT1622350.0143.542483
TCCGTCT4827250.0143.334381
GTCTATG604850.0143.094914
GTCTAGT595950.0143.069324
GTCTAGA484900.0143.04934
GTCTAAG512500.0142.945944
GTCTATA456250.0142.800254
GTCTAGG651600.0142.773094
GTCTAAT476500.0142.594364
GTCTAAA410650.0141.229374
GTCTATT543450.0141.13794
TCTAGTA182200.0140.52215
TCTAGTG212700.0139.768285
TCTATAG172650.0139.66255
TCTAAGG192050.0139.650745
TCTATGG231750.0139.090875
CTAGTGA60850.0139.036
Produced by FastQC (version 0.11.5)