FastQCFastQC Report
Sun 19 Mar 2023
SRR4895871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4804967
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG125730.26166672944892233No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG115830.2410630499647552No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG104390.21725435367193988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC98470.20493376957635714No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC97230.20235310669147155No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG93580.1947568006190261No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC92860.19325835120199578No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA92440.19238425570872805No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT91250.18990765181113625No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC90030.1873686125211682No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT88240.18364330077605112No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC87920.18297732325737096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT87530.18216566315647953No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC86230.17946012948684142No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT85610.17816979804439864No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT82840.1724049301483236No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT82480.17165570543980843No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT82090.170844045338917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC81620.16986589085835552No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT81430.1694704667066392No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT80310.16713954539125866No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT80190.1668898038217536No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC79270.1649751184555482No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC79200.1648294358733369No Hit
ATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATA78100.1625401381528739No Hit
TGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGA78060.16245689096303886No Hit
ACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTT77990.1623112083808276No Hit
GGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACG76360.15891888539505059No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT76080.15833615506620546No Hit
AAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATAC75650.157441247775479No Hit
TACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCT75340.1567960820542576No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC75300.15671283486442258No Hit
TGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAAC72400.15067741360138373No Hit
CTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCT72300.1504692956267962No Hit
ATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCC71260.1483048686910857No Hit
GTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATA71120.14801350352666315No Hit
ACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGT71090.14795106813428688No Hit
TCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCA70730.14720184342577172No Hit
CTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAA70120.14593232378078766No Hit
CACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTT69660.14497498109768497No Hit
TTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTAC69160.14393439122474722No Hit
TGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCAT68550.14266487157976318No Hit
ATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACA67170.13979284353045504No Hit
TTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGA66920.13927254859398616No Hit
TGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCC66470.13833601770834222No Hit
GTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAA66440.13827358231596595No Hit
TATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGAC66240.13785734636679087No Hit
CTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTT66130.13762841659474456No Hit
AGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTT66100.13756598120236832No Hit
CTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTT66020.13739948682269826No Hit
GTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAG65690.13671269750655934No Hit
CACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCT65470.13625483796246676No Hit
AAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTA65390.13608834358279673No Hit
TTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA64660.1345690823683076No Hit
AACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACT64190.13359092788774615No Hit
AGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGA63980.13315388014111232No Hit
AGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC63960.1331122565461948No Hit
TGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTT63760.1326960205970197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63640.13244627902751466No Hit
GGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACC63440.13203004307833957No Hit
ACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTA63040.13119757117998937No Hit
ACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCC62900.13090620601556682No Hit
AAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGA62790.13067727624352052No Hit
AGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTC62580.13024022849688666No Hit
TTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATG62470.13001129872484035No Hit
GAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTT61240.12745144763741353No Hit
TGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTAT61230.1274306358399548No Hit
TGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTG60430.1257656920432544No Hit
AGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTG59870.12460023138556416No Hit
TGCCTTCCTCCTTATCCTACAGTACAATCAGCAGTCTAGTTCTTTTCATT59490.12380938308213148No Hit
CAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAAT59390.12360126510754393No Hit
TACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAG59220.1232474645507451No Hit
CTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGC59170.12314340556345132No Hit
GCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAG59000.1227896050066525No Hit
GAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGG58970.12272716961427622No Hit
TTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTA58500.12174901513371475No Hit
GTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACT58490.121728203336256No Hit
CTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAG58160.12104141402011709No Hit
GTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT58030.12077086065315329No Hit
CTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAAT57970.12064598986840076No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC57770.12022975391922568No Hit
GATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGAT57760.12020894212176692No Hit
TGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAG57090.11881455169203035No Hit
TGCAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTC56630.11785720900892764No Hit
TAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATAT56470.11752422024958757No Hit
CACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTT55800.116129829819851No Hit
GACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTA55770.11606739442747474No Hit
GCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTA55770.11606739442747474No Hit
GATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATG55380.11525573432658331No Hit
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTT55130.11473543939011444No Hit
AGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTAC55120.11471462759265569No Hit
AAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAA55080.11463138040282066No Hit
CAGTACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTT54970.11440245063077435No Hit
AAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTG54930.11431920344093935No Hit
CCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCT54850.11415270906126931No Hit
TGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCT54680.11379890850447048No Hit
TTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTT54640.11371566131463545No Hit
CACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAAATGTT54560.11354916693496542No Hit
TTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTG54310.11302887199849657No Hit
CACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACA54250.11290400121374404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA54220.11284156582136776No Hit
TGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTA53780.11192584673318255No Hit
CTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTAC53630.11161366977130123No Hit
CTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTAT53400.11113499842974987No Hit
CAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCT53310.11094769225262109No Hit
TCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACT53290.11090606865770357No Hit
TGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCA52790.10986547878476585No Hit
TTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACC52500.10926193665846196No Hit
ATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGG52390.10903300688641566No Hit
TGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGC52240.10872082992453434No Hit
CCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCT51950.10811728779823045No Hit
GATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAG51930.10807566420331295No Hit
ACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTC51920.10805485240585419No Hit
ATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGA51820.10784673443126666No Hit
AGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCA51580.10734725129225653No Hit
ACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTAC51500.10718075691258651No Hit
TCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGC51390.1069518271405402No Hit
GTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTT51190.1065355911913651No Hit
TGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTTGACAC50840.1058071782803087No Hit
CTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGT50260.1046000940277009No Hit
TATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCT50150.10437116425565461No Hit
TACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTC49350.10270622045895424No Hit
TTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCT49150.10228998450977915No Hit
ATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATT49000.10197780754789784No Hit
ACTTGTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATG48980.10193618395298032No Hit
GTTATTATCACAAATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTG48850.10166563058601652No Hit
GGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATCAG48730.10141588901651147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA600850.0140.89548
TGGTATC601650.0140.743947
GTGGTAT601750.0140.698096
AAGCAGT602950.0140.377091
GTATCAA609050.0138.986629
CAGTGGT610250.0138.816124
AGCAGTG614400.0137.784712
GCAGTGG616600.0137.35153
AGTGGTA624400.0135.582765
TCAACGC597650.028.61667810-14
CAACGCA599400.028.5523510-14
ATCAACG598250.028.5470610-14
AGAGTAC600000.028.41819815-19
TACATGG600350.028.41122220-24
CGCAGAG601700.028.35226415-19
CAGAGTA603400.028.28431915-19
GTACATG603500.028.27247420-24
AGTACAT605700.028.10797120-24
GCAGAGT608100.028.04439715-19
GAGTACA602300.028.02755720-24
Produced by FastQC (version 0.11.5)