FastQCFastQC Report
Sun 19 Mar 2023
SRR4895870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210335
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCA2213500.0143.791342
TATCAGC2214000.0143.758851
TCAGCAG819150.0143.542433
TCAGCAT754800.0143.493943
TCAGCAA634850.0143.250983
CAGCAGT269350.0142.475854
CAGCATG276450.0142.462894
CAGCATA218800.0142.255454
CAGCATT249850.0142.155214
CAGCAAG236800.0142.053564
CAGCAAT215900.0141.931884
CAGCAGG312000.0141.414894
AGCAGTG92550.0141.198875
CAGCAGA226150.0141.197834
AGCAATG81550.0141.174265
AGCATGG97650.0141.05025
AGCATGT96450.0141.01355
AGCATAG78250.0140.779075
AGCATTG92550.0140.732095
AGCAGAT76050.0140.591225
Produced by FastQC (version 0.11.5)