FastQCFastQC Report
Sun 19 Mar 2023
SRR4895870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210335
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG84790.38360700979715745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC63870.28896072314830107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT27060.1224248812962741No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25120.11364793119594994No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG24700.1117477667412406No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC23630.10690687158281438No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG23010.10410186691157675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT22620.10233742848934663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273500.0140.839788
GTGGTAT274850.0140.252796
TGGTATC275400.0139.97277
GTATCAA276250.0139.41179
AAGCAGT277950.0138.762331
CAGTGGT280800.0137.34154
GCAGTGG286600.0134.536973
AGCAGTG286800.0134.418052
AGTGGTA297200.0129.705525
TCAACGC272900.028.56231110-14
ATCAACG272650.028.55681210-14
CAACGCA274050.028.46347410-14
AGAGTAC273300.028.41513315-19
CGCAGAG274800.028.3124315-19
TACATGG274750.028.3123420-24
CAGAGTA276450.028.13302815-19
GTACATG277750.028.02727120-24
GAGTACA274150.028.0171320-24
AGTACAT278050.027.92452820-24
GCAGAGT279200.027.871415-19
Produced by FastQC (version 0.11.5)