FastQCFastQC Report
Sun 19 Mar 2023
SRR4895869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423749
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG1427300.0143.636021
AGGATGA1429400.0143.4252
GGATGAT541850.0142.909643
GGATGAG482150.0142.520863
GGATGAA408550.0142.466023
GATGATG197800.0137.083194
GATGATA163700.0136.566164
GATGAGT172500.0136.444494
GATGAGA143550.0136.074514
GATGAAG145100.0135.563724
GATGAGG175350.0135.335464
GATGAAT155850.0134.852024
ATGAGTA53450.0134.30055
GATGAAA124350.0133.866584
ATGAGAG47350.0132.898975
ATGAGTG58350.0132.893885
ATGATAG57750.0132.653845
ATGATTG69150.0132.44185
ATGAATG56450.0132.137475
ATGATAT58000.0130.095845
Produced by FastQC (version 0.11.5)