FastQCFastQC Report
Sun 19 Mar 2023
SRR4895869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423749
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG42150.29604937387137764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC27430.19266036358936864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24100.16927140949703914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22490.15796323649744443No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG17690.12424942879678932No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG16760.11771737855478741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC16050.11273054449906549No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG15350.10781394754271996No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC15180.10661991685332177No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC14870.10444256677265445No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC14740.10352948448076171No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA14550.10219497959261079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA14470.10163308279759985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT14330.10064976340633076No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14310.10050928920757801No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT14290.1003688150088253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC257650.0140.839757
GGTATCA257900.0140.759058
GTGGTAT257900.0140.647386
AAGCAGT258200.0140.285521
GTATCAA259000.0140.077859
CAGTGGT261150.0138.911674
AGCAGTG261450.0138.64212
GCAGTGG264400.0137.149723
AGTGGTA272550.0133.034535
TCAACGC256000.028.61387310-14
ATCAACG255650.028.5741910-14
TACATGG255550.028.52902620-24
CAACGCA256850.028.52478410-14
GTACATG256000.028.46762520-24
CAGAGTA257950.028.34732215-19
AGAGTAC257550.028.34102615-19
CGCAGAG257750.028.31903615-19
AGTACAT257050.028.30092220-24
GAGTACA256400.028.20980320-24
GCAGAGT259750.028.13979715-19
Produced by FastQC (version 0.11.5)