FastQCFastQC Report
Sun 19 Mar 2023
SRR4895868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585965
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT586500.0143.864961
ATCCGTC586850.0143.779162
CGTCGTA25950.0143.167635
CCGTCGA65750.0143.123954
TCCGTCG240950.0142.954133
TCCGTCA167950.0142.885383
CCGTCTA48700.0142.817264
CGTCAAT18200.0142.813195
CGTCAAG15800.0142.632925
CCGTCAA48700.0142.373734
CGTCAGT21000.0142.28575
CGTCGAT23250.0142.141945
CCGTCAT60300.0142.089554
CCGTCAG56400.0142.085114
CGTCTAA13450.0141.858735
CGTCTAG16500.0141.818185
TCCGTCT181500.0141.65953
CGTCGGA20700.0141.565225
CGTCGAA20600.0141.553395
CCGTCTG64150.0141.418554
Produced by FastQC (version 0.11.5)