FastQCFastQC Report
Sun 19 Mar 2023
SRR4895868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585965
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61421.0481854718285222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGCCCGCGCGGCGTGAA24750.42238017629039276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCTCCTACGCCGTGGCTC17500.29865264990229795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGGGGGTCCCAAGGA17450.2977993566168628No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGCCGCAGCACCACTGAC16230.27697900045224544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14830.2530867884600616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCGCGATGTGCGCTTCGAG12650.21588320121508964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTTCTGCGCACCAAAGA11830.2018891913339534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT11320.19318559982251501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT10960.18704188816738201No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA10480.1788502726272047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA10420.17782632068468252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTCAGCTGTCAATGTG10420.17782632068468252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10090.17219458500081064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTCAGGTGGTGTGCAACT9670.16502692140315547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACCCCGTGGTCTCCTACG9450.16127243094724086No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC9180.15666464720589113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCACTCTGCCGCCGCAGCAC8620.1471077624090176No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7400.12628740624440027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA7390.12611674758731325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTCAAAAAAAAAAAAAAA7340.1252634543018781No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGTCTCACTCCAGCAGAC7330.12509279564479106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCACCGGAACCGGATTGAG7050.12031435324635431No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCACTCTGCCGCCGCAGC7010.1196317186180062No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCGCAAAAAAAAAAAAA6760.1153652521908305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGACCCCCGCTTCCAGGA6470.11041615113530671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC6090.10393112216599967No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5960.10171255962386833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT293400.0142.674851
TGGTATC295300.0141.976297
GGTATCA295300.0141.854398
GTGGTAT295750.0141.760276
GTATCAA295750.0141.589869
CAGTGGT296800.0141.23454
AGCAGTG297250.0140.899582
AGTGGTA297550.0140.830115
GCAGTGG301000.0139.239873
GTATCCA752.6557283E-548.09
GGTATCC1201.0021411E-536.08
CAACGCA294750.028.68274910-14
TCAACGC294850.028.6583710-14
ATCAACG294800.028.62903810-14
CGCAGAG294550.028.61422515-19
TACATGG293550.028.59887520-24
AGTACAT293100.028.57400120-24
GTACATG294200.028.54058520-24
AGAGTAC294450.028.491915-19
GCAGAGT295050.028.48276715-19
Produced by FastQC (version 0.11.5)