FastQCFastQC Report
Sun 19 Mar 2023
SRR4895867_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895867_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692694
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG606550.0143.620153
TGTTCTA1698400.0143.512482
GTTCTAA504500.0143.200793
GTTCTAT585450.0142.930053
GTGTTCT1707450.0142.751831
TCTAGTA64000.0140.51255
TTCTAGT222550.0140.376544
TTCTATG224950.0140.095144
TTCTAGG222450.0139.986534
TCTAAGG68600.0139.591845
TCTATGG80100.0139.505625
TTCTAAG189850.0139.486974
TCTAGTG71200.0139.044945
CTAGTAT23150.0139.023766
TCTAGGT75550.0138.949045
TTCTAGA165500.0138.30094
TCTATGA60200.0137.780725
TCTATAG60000.0137.645
CTATGAG21400.0137.607486
TCTAAGA50050.0137.526465
Produced by FastQC (version 0.11.5)