FastQCFastQC Report
Sun 19 Mar 2023
SRR4895866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359723
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGACA1364050.0143.53911
GGACACT459300.0143.517623
AGGACAC1366100.0143.32372
GACACTA138750.0143.27714
GACACGT185750.0143.189594
GACACGA143400.0143.099824
GACACTG158450.0142.822134
GGACACA390050.0142.711433
GACACTT155650.0142.523334
GACACAT141250.0142.423394
GGACACG517450.0142.20863
GACACAG130400.0141.24284
ACACGTA56250.0141.059525
ACACGTG59900.0140.998545
ACACTAG50250.0140.7085
ACACGGT62850.0140.10855
ACACGAT50550.0139.872945
GACACGG177200.0139.655874
ACACGAG47850.0139.338915
ACACTGA44300.0139.290185
Produced by FastQC (version 0.11.5)