FastQCFastQC Report
Sun 19 Mar 2023
SRR4895866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359723
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG34000.250050929490786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23050.16951982131654758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19190.14113168638024068No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG18790.1381899107391726No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG16510.12142178958508461No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT14280.1050213903861301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105500.0140.517628
TGGTATC105850.0140.053017
GTGGTAT105850.0139.984996
AAGCAGT107650.0137.402051
CAGTGGT108300.0136.828264
GTATCAA108500.0136.499659
AGCAGTG110200.0134.474092
GCAGTGG111750.0132.60893
AGTGGTA125150.0118.516635
GTCGTAG400.006215093754.009241
CAACGCA104300.028.63395910-14
TCAACGC104500.028.59293710-14
ATCAACG104550.028.55171810-14
TACATGG105950.028.22880420-24
GTACATG106300.028.1358620-24
AGAGTAC106150.028.13491815-19
CGCAGAG106350.028.02785115-19
GAGTACA106100.027.86316920-24
AGTACAT107500.027.83517820-24
CAGAGTA107900.027.77202815-19
Produced by FastQC (version 0.11.5)