FastQCFastQC Report
Sun 19 Mar 2023
SRR4895865_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895865_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4217965
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC4226350.0143.71312
GAGATCA1226800.0143.654753
AAGAGAT4230900.0143.558561
GAGATCT1427800.0143.300083
AGATCGA447850.0143.197174
AGATCGT584000.0143.039374
AGATCAG420150.0143.007084
AGATCAT439700.0142.985784
AGATCTA408250.0142.854644
GATCGAT160400.0142.833925
AGATCTG486000.0142.667664
GAGATCG1576500.0142.644593
GATCATG153850.0142.363055
GATCAGT147500.0142.048465
AGATCAA355300.0141.893484
GATCATA124550.0141.630875
GATCGTG197400.0141.557245
GATCTAT139750.0141.321935
ATCGAGT56300.0141.18756
GATCTAG146050.0141.141715
Produced by FastQC (version 0.11.5)