FastQCFastQC Report
Sun 19 Mar 2023
SRR4895865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4217965
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG172110.4080403701785103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC114280.2709363401545532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85290.20220651427880504No Hit
GTGAACCCCGTGGTCTCCTACGCCGTGGCTCTCAGCTGTCAATGTGCACT48530.11505548291652491No Hit
GGGGGTCCCAAGGACCACCCCTTGACCTGTGATGACCCCCGCTTCCAGGA47570.11277950386027384No Hit
GGCGTGAACCCCGTGGTCTCCTACGCCGTGGCTCTCAGCTGTCAATGTGC46770.11088285464673131No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT46270.10969744888826721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC45920.10886766485734235No Hit
AGCACCACTGACTGCGGGGGTCCCAAGGACCACCCCTTGACCTGTGATGA45230.1072318049106619No Hit
CGCAGCACCACTGACTGCGGGGGTCCCAAGGACCACCCCTTGACCTGTGA43790.1038178363262853No Hit
CGCCGCAGCACCACTGACTGCGGGGGTCCCAAGGACCACCCCTTGACCTG43130.10225310072511269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA426950.0140.828988
TGGTATC428800.0140.338937
GTGGTAT428850.0140.27226
GTATCAA432300.0139.01959
AAGCAGT436200.0137.840771
CAGTGGT439900.0136.786254
GCAGTGG446850.0134.610433
AGCAGTG448000.0134.280962
AGTGGTA496000.0121.3121955
TCAACGC424150.028.57240510-14
CAACGCA425900.028.45838510-14
ATCAACG425700.028.4379310-14
TACATGG427200.028.28077120-24
CGCAGAG429400.028.16941515-19
AGAGTAC429900.028.11990215-19
GTACATG433850.027.8738420-24
GAGTACA430100.027.8624220-24
CAGAGTA435550.027.80472215-19
AGTACAT435000.027.72401420-24
GCAGAGT440500.027.49227115-19
Produced by FastQC (version 0.11.5)