FastQCFastQC Report
Sun 19 Mar 2023
SRR4895864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2978530
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC2993550.0143.274811
ACTCACC2999350.0142.997742
CTCACCG1135750.0142.946433
TCACCGA314850.0142.603824
TCACCGT407450.0142.426064
TCACCGG392700.0141.486944
TCACCTG343600.0140.89754
CTCACCT1010550.0140.650123
TCACCAG296100.0140.618854
TCACCTA293700.0140.493164
CTCACCA875150.0140.387073
CACCGTA124800.0140.306495
CACCGAT114850.0139.547785
CACCGTG141450.0139.061375
TCACCAT312200.0138.533084
ACCGATA34100.0138.086796
ACCGATG40050.0138.066226
ACCGTGA41600.0137.768046
CACCGGT140950.0137.613575
CACCGAG107800.0137.586935
Produced by FastQC (version 0.11.5)