FastQCFastQC Report
Sun 19 Mar 2023
SRR4895863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482953
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA2486600.0143.788622
CACGCAT886900.0143.618443
TTCACGC2490950.0143.537521
ACGCATG322150.0143.485954
CACGCAG882000.0143.436743
ACGCAGT302550.0143.428864
ACGCATA254150.0143.348424
ACGCAGA238950.0142.824864
ACGCAAT250400.0142.821084
ACGCATT294900.0142.803674
ACGCAGG323100.0142.707524
CACGCAA715550.0142.701983
ACGCAAG256250.0142.679414
CGCATTA85400.0141.807955
CGCAGTA91300.0141.634175
CGCATGT112300.0141.627795
CGCAGTT105850.0141.551255
CGCAAGT85000.0141.543535
CGCAATG90450.0141.532335
CGCATAT87400.0141.446215
Produced by FastQC (version 0.11.5)