FastQCFastQC Report
Sun 19 Mar 2023
SRR4895863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482953
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG97070.39094578109211087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC61160.24631960411654993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59480.23955346718202075No Hit
TGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGC33980.13685317442577447No Hit
GGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCT30050.12102524695392945No Hit
GAGACATTCCTGCGCATGGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGG29080.11711860836673106No Hit
AAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGA28210.11361471602563561No Hit
AACCATGACGCACTGCTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAA27880.11228565341349596No Hit
CGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAA27260.1097886266876578No Hit
TGGAAGACGGCAGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGC26930.10845956407551814No Hit
CGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACACAAACTC25990.10467374936215064No Hit
AGCCGCCGGACTGGGCAGATCCTCAAGCAGACCTACAGCAAGTTTGACAC25750.1037071583715036No Hit
AGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCG25510.10274056738085659No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC25060.10092820927339341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA350700.0140.406948
GTGGTAT351100.0140.267476
TGGTATC351350.0140.147197
AAGCAGT353850.0139.046131
GTATCAA355350.0138.468299
CAGTGGT357100.0137.959374
AGCAGTG362050.0135.933942
GCAGTGG362800.0135.732333
AGTGGTA376350.0130.894935
TCAACGC348300.028.54361710-14
ATCAACG348150.028.53937110-14
CAACGCA349900.028.42544210-14
TACATGG350600.028.29475620-24
AGAGTAC351200.028.23411615-19
GTACATG351300.028.22198120-24
CGCAGAG352200.028.15804115-19
CAGAGTA353750.028.08758215-19
GAGTACA349950.028.05927520-24
AGTACAT353150.028.04967320-24
GCAGAGT354450.027.99554815-19
Produced by FastQC (version 0.11.5)