FastQCFastQC Report
Sun 19 Mar 2023
SRR4895862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353025
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA4380800.0143.085712
GTGGTAG1646550.0142.748893
GTGGTAA1237950.0142.382643
TGGTGGT4420650.0141.795851
GTGGTAT1511250.0141.62693
TGGTATA407650.0139.972534
TGGTAGA427100.0139.953634
TGGTATG559250.0139.853974
TGGTAGG604850.0139.737964
TGGTAAG458550.0139.712974
TGGTAGT541550.0139.133484
TGGTAAT427800.0138.697984
TGGTAGC111650.0137.937734
GGTAGTA146950.0136.062155
GGTAGTC66050.0135.932925
GGTATTG181900.0135.014395
GGTATAG149450.0134.364215
GGTATGC66950.0134.213135
GGTATGG202850.0134.096655
GGTAATC55450.0133.741655
Produced by FastQC (version 0.11.5)