FastQCFastQC Report
Sun 19 Mar 2023
SRR4895861_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895861_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669483
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA3681000.0143.548072
TGGCTTC3687150.0143.308641
GCTTCAG1397950.0143.175843
GCTTCAA1028700.0142.628083
GCTTCAT1267200.0142.056723
CTTCAGT472050.0141.757774
CTTCAGA379800.0140.777164
CTTCAAG392000.0140.583574
CTTCATG480600.0139.775194
CTTCAAT360800.0138.791494
CTTCAGG546500.0138.334764
TTCAGAG136200.0138.026345
CTTCATA372300.0137.308524
TTCAGTA136000.0137.011675
TCAGTGA47900.0136.484256
TTCATGG186150.0135.91615
TTCAGGA148700.0135.86545
TCATGGA49550.0135.717366
TCATAGG52100.0135.017186
TTCAGGT189950.0134.902795
Produced by FastQC (version 0.11.5)