FastQCFastQC Report
Sun 19 Mar 2023
SRR4895860_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895860_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7532943
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAT2722100.0142.428283
AACAATG1009700.0141.44664
AACAAGT998700.0140.639864
AACAAGG1139800.0140.272464
ACAATGG375600.0139.935535
ACAAGTG347050.0139.891665
ACAATTG341000.0139.438725
TGGAACA7812050.0138.853851
ACAAGTA298800.0138.481375
ACAATAG291400.0138.291815
GGAACAA7849550.0138.190492
ACAATGA275550.0137.179635
ACAAGAG292100.0136.875855
ACAAGAT275350.0135.605775
GAACAAA2010950.0135.531813
CAAGTAG106400.0135.473146
ACAAGGG418000.0135.369785
ACAAGGT396300.0135.296955
ACAAGTT348000.0134.668435
AACAAGA829150.0134.595124
Produced by FastQC (version 0.11.5)