FastQCFastQC Report
Sun 19 Mar 2023
SRR4895860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7532943
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGGTGTGCAACTACCGC314310.41724728303400144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG223310.29644456356566085No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211950.2813641361682944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT162390.21557311664245965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC151230.2007581897274412No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG110960.14729966760667113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC106430.1412860816814889No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG102990.13671947338510326No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG91330.12124079526421479No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA90720.12043101879305339No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC88060.11689986237782499No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT88050.11688658735370758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTACCGCGATGTGCGCTT87590.11627593624430717No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC86410.11470948339845397No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT86140.11435105774728416No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT86120.11432450769904937No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC85640.11368730654141417No Hit
ATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCT83100.11031545041559454No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT81100.10766044559211453No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT81010.10754097037505793No Hit
CCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTC80460.10681084404860093No Hit
AAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACAATC80080.10630639313213973No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT80020.10622674298743533No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT79060.10495234067216491No Hit
AATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTC77020.1022442357522153No Hit
TTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATAC76970.10217786063162831No Hit
GAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTC76950.10215131058339351No Hit
GTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTGT76910.10209821048692391No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT76870.1020451103904543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1894600.0141.049857
GTGGTAT1896000.0140.903926
GGTATCA1896450.0140.897058
GTATCAA1899900.0140.633649
AAGCAGT1906000.0140.145321
CAGTGGT1919500.0139.18054
AGCAGTG1922300.0138.968352
GCAGTGG1941200.0137.635773
AGTGGTA1972150.0135.442235
TCAACGC1884150.028.65073410-14
ATCAACG1884750.028.6026510-14
CAACGCA1890100.028.57349410-14
TACATGG1891450.028.44347220-24
AGAGTAC1891150.028.42894615-19
CAGAGTA1894550.028.42201215-19
CGCAGAG1892900.028.42092315-19
GTACATG1895100.028.38564920-24
AGTACAT1897850.028.28836820-24
GCAGAGT1908600.028.2052415-19
GAGTACA1891600.028.15346320-24
Produced by FastQC (version 0.11.5)