FastQCFastQC Report
Sun 19 Mar 2023
SRR4895859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6018456
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG6041500.0143.450271
GAAGAGA6063900.0142.920362
AAGAGAT2062550.0142.079713
AAGAGAG2322600.0141.57863
AAGAGAA1734100.0139.449073
AGAGATG854600.0129.163284
AGAGAGG989250.0127.761954
AGAGATA661950.0127.4232254
AGAGAGT827900.0126.997664
AGAGAGA703400.0126.393794
GAGAGTG295350.0126.252635
GAGATGA240900.0125.8577355
GAGATTG280900.0125.74985
GAGATTA205550.0125.610025
GAGATGG321050.0125.4530645
AGAGAAG738050.0125.200994
GAGAGTA246100.0124.690495
GAGATAG246550.0124.46295
GAGAGGT340850.0124.227965
GAGATGT296900.0123.871735
Produced by FastQC (version 0.11.5)