FastQCFastQC Report
Sun 19 Mar 2023
SRR4895858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4923531
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA4955650.0143.065172
TTCACAG1821800.0143.044423
TCTTCAC4970250.0142.644931
TCACAGT629550.0142.46834
TCACATG637900.0142.37554
TCACAGA492350.0142.216744
TCACAGG670100.0141.884124
TTCACAT1736750.0141.559023
CACATGG237200.0141.268955
TCACATA495500.0140.426254
CACAGTG223400.0140.262225
TCACAAG520500.0140.182954
CACAGTA187200.0139.923895
CACAGGT231300.0139.798485
CACAGAT173550.0139.644735
CACATTG222100.0139.5925
TTCACAA1432900.0139.573993
TCACAAT497700.0139.053254
CACATAG186950.0139.032645
TCACATT584150.0138.565224
Produced by FastQC (version 0.11.5)