FastQCFastQC Report
Sun 19 Mar 2023
SRR4895857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4571737
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA4578800.0143.77612
CGTCTAG1679100.0143.679353
CGTCTAT1538900.0143.612613
CGTCTAA1352200.0143.590943
TCCGTCT4593650.0143.31131
GTCTAGA462600.0143.113784
GTCTATG564500.0143.108124
GTCTAAG494100.0142.980914
GTCTAGT580750.0142.947144
GTCTAGG611550.0142.78834
GTCTATA434550.0142.774844
GTCTAAT464900.0142.436744
GTCTAAA385400.0141.66574
GTCTATT524950.0141.34014
TCTAGTG211400.0140.731315
TCTAAGG191000.0140.11825
TCTATGG212450.0140.103535
TCTAGGT210150.0140.026635
TCTAGTA172000.0139.814875
TCTATGA157350.0139.379385
Produced by FastQC (version 0.11.5)