FastQCFastQC Report
Sun 19 Mar 2023
SRR4895857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4571737
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG389300.8515362979104003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC273280.5977596699022713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT90970.19898344983536892No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71320.15600197474176664No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG68650.1501617437748497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA66210.144824603865008No Hit
CAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTG62540.13679702047602477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC53250.11647651647502907No Hit
CCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTT52610.11507661092490665No Hit
AAAACTCTCCTTTTCCTTACCATACCACTTTGACACGCTTCAAGGATATA51420.11247366154264779No Hit
AAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGT51200.1119924440097932No Hit
CTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGC51190.11197057048557256No Hit
CCTTACCATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTG50440.11033005616902283No Hit
ATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTC49860.10906139176422441No Hit
AATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCT48800.10674279819683416No Hit
GGAATGGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACT48530.10615221304287627No Hit
CATACCACTTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCC48150.10532101912249109No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC47310.10348364308795542No Hit
GGAAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCT47010.10282743736133554No Hit
AAAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTT46230.10112130247212382No Hit
GTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCACTTT45790.10015886740641468No Hit
AAATTAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTT45770.10011512035797335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA509950.0140.567878
TGGTATC512750.0139.912617
GTGGTAT512950.0139.872096
GTATCAA514950.0139.147089
AAGCAGT519750.0138.070821
CAGTGGT528550.0135.831224
AGCAGTG530300.0135.330152
GCAGTGG532700.0134.747483
AGTGGTA592500.0121.2762455
TCAACGC506950.028.592410-14
ATCAACG507900.028.50489610-14
CAACGCA509100.028.4914510-14
TACATGG512650.028.24920820-24
AGAGTAC513650.028.20262315-19
GTACATG513950.028.15814220-24
CGCAGAG515750.028.0654515-19
CAGAGTA518100.028.00485615-19
AGTACAT517350.027.91463920-24
GAGTACA514050.027.85292820-24
GCAGAGT527650.027.48980715-19
Produced by FastQC (version 0.11.5)